Structure of leaf & green plastid , Mechanism of photosynthesis in green plants
Green leaves are the main sites of photosynthesis as they contain the green plastids in the higher plants , Green herbaceous stems may participate , to some extent , in this process as they contain chlorenchymatous tissues which contain green plastids .
Structure of green plastid
Green plastid ( Chloroplast ) appears under the light microscope as a homogeneous mass , having the shape of a convex lens , After studying the green plastid under the electron microscope , it was found that it consists of Double thin external membrane , Matrix ( Stroma ) , Starch granules and Grana .
Green plastid consists of : Double thin external membrane & its thickness is about 10 nm , Matrix ( Stroma ) is a colourless protein substance .
Starch granules are large in number , They are small in size and a temporary products of the photosynthesis process as they will soon change back into a soluble sugar to be translocated under certain conditions to other organs of the plant .
Grana are located in the stroma , They have disc-shaped structures , each granum is about 0.5 micron in diameter and about 0.7 micron in thickness , Each granum is a pile ( mass ) of 15 hollow discs or more that are arranged above each other .
The margin of some discs of a granum extends to meet the margin of another disc in the neighbouring granum to increase the surface area of grana that are exposed to the light , They contain the pigments which absorb the light energy needed for photosynthesis .
Main four pigments in chloroplates :
- Chlorophyll A : Its colour is blue-green , ratio is 70 % .
- Chlorophyll B : Its colour is Yellow-green , ratio is 70 % .
- Xanthophyll : Lemon-yellow , ratio is 25 % .
- Carotene : Orange-yellow , ratio is 5 % .
The green colour dominates over the other colours of pigments due to the high ratio of the chlorophyll pigments .
The importance of chlorophyll is the absorption of light energy needed for the photosynthesis process , The structure of chlorophyll is complicated structure and the molecular formula of chlorophyll ( A ) is C55H72O5N4Mg .
It is believed that there is a relation between the presence of Mg atom in the centre of chlorophyll molecule and the ability of chlorophyll to absorb light .
Structure of the leaf
Examination of T.S. passing through the midrib of a dicotyledonous plant leaf , the leaf consists of three main tissues which are upper and lower epidermis , Mesophyll tissue and vascular tissue .
Upper and lower epidermis
Each of the upper and lower epidermis consists of one row of adjacent barrel-shaped parenchyma cells with no chlorophyll , Stomata spreed throughout the epidermal layer , the external wall of epidermis is coated by a layer of cutin , except the stomata .
It lies between the upper and lower epidermal layers and is transversed by veins , It consists of two layers which are palisade layer and spongy layer .
Palisade layer is perpendicular to the upper epidermis of the leaf surface , It consists of one row of cylindrical and elongated parenchyma cells , It possesses many chloroplasts ( especially in the upper part of cells ) to receive the highest light intensity .
Spongy layer is located below the palisade tissue , It consists of irregular-shaped and loosely-arranged parenchyma cells with wide intercellular spaces , The cells of this tissue contain a lower number of chloroplasts than the cells of palisade tissue .
It consists of numerous vascular bundles which extends through the veins and venules , The midrib contains the main vascular bundle of the leaf , The vascular bundle consists of Xylem vessels and Pheloem .
Xylem vessels : several vertical rows that are separated by a thin-walled cells called xylem parenchyma .
Phloem : It lies toward the lower epidermis , It translocates the dissolved organic food from the mesophyll where it has been manufactured to the other parts of plant .
Mechanism of photosynthesis
Source of oxygen evolved during photosynthesis : The American scientist Van Neil was the first person who pointed out the source of oxygen evolved in photosynthesis by studying this process in the green and purple sulphur bacteria .
Green and purple sulphur bacteria
Green and purple sulphur bacteria are autotrophic as they contain bacteriochlorophyll which is simpler in structure than the normal chlorophyll .
Van Neil assumed that : Light decomposed hydrogen sulphide into hydrogen and sulphur .
12H2S → 12H2 + 12 S ↓
The resulted hydrogen was used in certain dark reaction to reduce CO2 into carbohydrates .
12H2 + 6 CO2→ C6H12O6 + 6 H2O ↓
So , the general equation for photosynthesis in sulphur bacteria is :
6 CO2 + 12H2S→ C6H12O6 + 6 H2O + 12 S ↓
Van Neil assumed that :
12H2O → 12H2 + 6 O2 ↑
The resulted hydrogen was used in certain dark reactions to reduce CO2 into carbohydrates .
12H2 + 6 CO2 → C6H12O6 + 6 H2O
So , the general equation for photosynthesis in green plants is :
6 CO2 + 12H2S→ C6H12O6 + 6 H2O + 6 O2 ↑
Consequently , Van Neil proposed that : the released of oxygen comes from water in the green plants , a case which is similar to sulphur that is released from hydrogen sulphide by the sulphur bacteria .
To confirm that water is the source of oxygen gas that is evolved from the photosynthesis process , In 1941 , a group of scientists at California University verified the theory of Van experimentally by using a green agla called Chlorella and provided it with all conditions favourable for photosynthesis , The result : The source of evolved oxygen from photosynthesis is water and not CO2 gas .